Conifer needles (lyophilized)
Extract molecules (DNA, RNA, protein, chemicals)
Wet final product
Sample sizes: 10 to 100 mg.
Notes on the protocol:
One of these Bullet Blenders
Bullet Blender Blue
Bullet Blender Storm 24
Bullet Blender 24 Gold
NAVY bead lysis kit
0.9 - 2.0 mm stainless steel blend
(SSB14B) use 100ul beads
3.2 mm stainless steel beads
(SSB32) use 3 beads
Cut the sample into appropriately sized pieces. For larger samples, we recommend cutting the material into long, thin strips for faster homogenization.
Place the sample in the tube with the beads.
Set the controls for Speed 10 and Time 2. Press Start.
After the run, remove the tubes from the instrument and visually inspect the samples. If homogenization is incomplete, repeat the homogenization step.
Proceed with your downstream application.